Edinburgh Instruments now supplies a microscope attachment, which in combination with the FLS980 spectrometer, enables wide-field fluorescence measurements and specific points measurements. The attachment allows the excitation light (illumination) and the detected emission to be tuned as required.
The FLS980 spectrometer with microscope attachment incorporates comprehensive software, which controls the instrument and automated microscope stage, while containing analysis routines to scrutinise data. The microscope attachment is available for both upright and inverted microscopes.
Imaging cameras are available spanning the spectrum from the visible to the near-infrared, up to 1,700nm. Excitation can be provided by halogen lamps (wide-field excitation), picosecond pulsed diode lasers and LEDs, supercontinuum sources and Nd:YAG lasers (lasers and semiconductor sources provide point source illumination).
Steady-state emission spectra and fluorescence lifetime measurements can be obtained from specific spots on a sample when using appropriate lasers. Lasers can achieve a spot size of 1mm (objective dependent).
The FLS980, microscope control, and data analysis are all carried out using a single software package. The spot measurements are intuitively displayed showing the automatic point-source grid-like measurement.
